MtoZ Biolabs offers integrated protein lactylation modification analysis service, utilizing advanced Thermo Orbitrap Exploris™ 240 mass spectrometer and Nano-LC system. This service provides highly accurate identification of lactylation sites and in-depth analysis of their dynamic changes across diverse conditions. Tailored for functional proteomics, metabolic studies, and disease research, it delivers critical insights into the regulatory roles of lactylation, setting the stage for deeper exploration of its biological significance.
Protein lactylation is an emerging post-translational modification (PTM) that involves the formation of ester bonds between lactate molecules and amino acids with free amino groups. This modification serves as a metabolic sensor, reflecting changes in cellular metabolic states driven by endogenous lactate. Understanding this modification is pivotal for elucidating its role in metabolism, protein regulation, and disease progression.
MtoZ Biolabs’ Protein Lactylation Modification Analysis Service Workflow
To facilitate the in-depth study of lactylation modifications and their implications in disease mechanisms, MtoZ Biolabs has offered high-quality protein lactylation analysis services and solutions. This workflow leverages cutting-edge technologies and expertise to deliver reliable and comprehensive results. The steps are detailed below:
1. Protein Sample Preparation
The quality of sample preparation plays a pivotal role in protein lactylation modification analysis service, as protein integrity and purity directly affect analytical accuracy. Using optimized protocols, MtoZ Biolabs isolates proteins from a variety of complex biological samples, including cells, tissues, and body fluids, ensuring the structural stability and abundance of target proteins.
2. Protein Digestion
Specific proteolytic enzymes, such as trypsin or chymotrypsin, are employed under carefully optimized conditions to achieve efficient cleavage while preserving lactylation sites. The resulting peptides are of appropriate length, ensuring excellent solubility and ionization properties for high-efficiency downstream analysis.
3. Peptide Enrichment
Enrichment of lactylated peptides is a critical step, given the low abundance of these modifications. The protein lactylation modification analysis service provided by MtoZ Biolabs employs advanced targeted enrichment strategies, including the use of affinity-based antibodies against lactylation and specialized solid-phase extraction methods, to selectively isolate lactylated peptides.
4. Liquid Chromatography Separation
Post-enrichment, peptide mixtures may still contain components requiring further refinement. High-performance liquid chromatography (HPLC) or Nano-LC techniques are applied to achieve precise separation. Nano-LC, in particular, is advantageous for handling minute sample volumes, providing high-resolution separation that reduces co-elution artifacts. This step ensures improved signal-to-noise ratios during mass spectrometry analysis and facilitates the accurate identification of peptide sequences and modification sites.
5. Mass Spectrometry Analysis
Advanced mass spectrometry systems, such as the Thermo Orbitrap Exploris™ 240, are employed to conduct high-resolution analyses of lactylated peptides. The systems measure the mass-to-charge ratios of peptides and their fragmentation patterns, enabling precise identification of amino acid sequences and lactylation sites. Quantitative data are also obtained to assess the abundance of lactylation modifications under various conditions.
6. Data Processing and Bioinformatics Analysis
The datasets generated by mass spectrometry are subjected to in-depth analysis using sophisticated bioinformatics tools. Customized protein lactylation analysis identifies lactylation modification sites, determines modification patterns, and elucidates their biological significance. Additionally, comprehensive statistical evaluations and functional pathway analyses are performed to provide actionable insights into the roles of lactylation modifications in biological systems and disease mechanisms.
Applications
At MtoZ Biolabs, our advanced protein lactylation modification analysis service empowers research across the following key areas:
1. Protein Structure and Functional Dynamics
Lactylation can alter protein stability, activity, and molecular interactions by modifying their three-dimensional structure. MtoZ Biolabs helps researchers identify lactylation sites and analyze their dynamic changes, uncovering key mechanisms regulating protein functions.
2. Mechanistic Insights into Disease Pathogenesis
Protein lactylation modification analysis service are increasingly recognized for their involvement in the molecular pathology of diverse diseases. For example, in cancer, metabolic disorders, and neurological conditions, lactylation has been implicated in modulating the activity of essential proteins, thereby influencing disease progression. Through its comprehensive research on lactylation, MtoZ Biolabs establishes a molecular framework that facilitates a deeper understanding of disease mechanisms and aids in the identification of novel therapeutic targets.
3. Development of Novel Drugs
The specificity and dynamic nature of lactylation make it a promising drug target. Through detailed analysis of the distribution of lactylation and its pathological alterations, MtoZ Biolabs supports researchers in identifying novel molecular markers, thereby providing a robust basis for the development of precision therapeutic strategies targeting lactylation.
4. Dynamic Regulation of Biological Systems
Protein lactylation modification analysis service provide insights into its role in modulating biological system dynamics, particularly under conditions of cellular stress, metabolic reprogramming, and organismal development. Through detailed analyses, this modification can be harnessed to advance our understanding of complex biological systems and to develop new strategies for synthetic biology and other applied fields.
Why Choose MtoZ Biolabs?
Our primary advantages include:
1. Advanced Analysis Platform
MtoZ Biolabs' advanced customized protein lactylation analysis platform guarantees reliable, fast, and highly accurate analysis service.
2. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all protein lactylation modification analysis service data, providing clients with a comprehensive data report.
What Could Be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
5. Raw Data Files
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption.
Interested in learning more about how our protein lactylation modification analysis service can enhance your research?
Media Contact
Company Name: MtoZ Biolabs
Contact Person: Terry
Email: Send Email
Phone: +1-857-362-9535
Address:155 Federal Street, Suite 700
City: Boston
State: MA 02110
Country: United States
Website: https://www.mtoz-biolabs.com/
